svm classification model using a linear kernel Search Results


anti human anti ido1  (Sino Biological)
93
Sino Biological anti human anti ido1
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Anti Human Anti Ido1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human anti ido1 - by Bioz Stars, 2026-03
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segmented flow analyzer model san plus analyzer  (Skalar Analytical)
99
Skalar Analytical segmented flow analyzer model san plus analyzer
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Segmented Flow Analyzer Model San Plus Analyzer, supplied by Skalar Analytical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multinomial logistic regression function mnrfit  (MathWorks Inc)
90
MathWorks Inc multinomial logistic regression function mnrfit
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Multinomial Logistic Regression Function Mnrfit, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multinomial logistic regression function mnrfit/product/MathWorks Inc
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matlab-based classification model effectiveness analysis tool roca  (MathWorks Inc)
90
MathWorks Inc matlab-based classification model effectiveness analysis tool roca
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Matlab Based Classification Model Effectiveness Analysis Tool Roca, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matlab-based classification model effectiveness analysis tool roca/product/MathWorks Inc
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matlab-based classification model effectiveness analysis tool roca - by Bioz Stars, 2026-03
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2d-fingerprint descriptor ecfp_4  (SciTegic Inc)
90
SciTegic Inc 2d-fingerprint descriptor ecfp_4
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
2d Fingerprint Descriptor Ecfp 4, supplied by SciTegic Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2d-fingerprint descriptor ecfp_4/product/SciTegic Inc
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contraa 700 highresolution continuum-source atomic absorption spectrometer  (Endress+Hauser inc)
90
Endress+Hauser inc contraa 700 highresolution continuum-source atomic absorption spectrometer
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Contraa 700 Highresolution Continuum Source Atomic Absorption Spectrometer, supplied by Endress+Hauser inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/contraa 700 highresolution continuum-source atomic absorption spectrometer/product/Endress+Hauser inc
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model bcs class ii compound  (Bristol Myers)
90
Bristol Myers model bcs class ii compound
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Model Bcs Class Ii Compound, supplied by Bristol Myers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stereo camera model 3-dx  (Nidek CO)
90
Nidek CO stereo camera model 3-dx
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Stereo Camera Model 3 Dx, supplied by Nidek CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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soft independent modeling class analogy (simca) software (version 17.0  (Umetrics)
90
Umetrics soft independent modeling class analogy (simca) software (version 17.0
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Soft Independent Modeling Class Analogy (Simca) Software (Version 17.0, supplied by Umetrics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/soft independent modeling class analogy (simca) software (version 17.0/product/Umetrics
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soft independent modeling class analogy (simca) software (version 17.0 - by Bioz Stars, 2026-03
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classification learner app  (MathWorks Inc)
90
MathWorks Inc classification learner app
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Classification Learner App, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/classification learner app/product/MathWorks Inc
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classification learner app - by Bioz Stars, 2026-03
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flame atomic absorption spectrometer (faas) zeenit 700  (Endress+Hauser inc)
90
Endress+Hauser inc flame atomic absorption spectrometer (faas) zeenit 700
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Flame Atomic Absorption Spectrometer (Faas) Zeenit 700, supplied by Endress+Hauser inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flame atomic absorption spectrometer (faas) zeenit 700/product/Endress+Hauser inc
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atr spectrophotometer bruker alpha  (Bruker Corporation)
90
Bruker Corporation atr spectrophotometer bruker alpha
<t>IDO1</t> inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.
Atr Spectrophotometer Bruker Alpha, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atr spectrophotometer bruker alpha/product/Bruker Corporation
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atr spectrophotometer bruker alpha - by Bioz Stars, 2026-03
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Image Search Results


IDO1 inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.

Journal: Frontiers in Immunology

Article Title: Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model

doi: 10.3389/fimmu.2021.781185

Figure Lengend Snippet: IDO1 inhibitor mixed with MSCs improved the efficacy of MSCs to promote cartilage regeneration in OA animal model. (A) The levels of IL-1β in the OA animal model after one month of model establishment compared to normal control. (B) The levels of TNF-α in the OA animal model after one month of model establishment compared to normal control. (C) The levels of IFN-γ in the OA animal model after one month of model establishment compared to normal control. (D) The schematic diagram presented the performed strategy for stimulated MSCs to be used in the cartilage repair for the OA model. (E) HE staining for knee joints of OA animal model after one month that presented the initiation of cartilage degeneration compared to normal control groups. (F) Safranin-O staining for the cartilage of OA model after two months of treatment with Epacadostat-treated MSCs, IDO1-treated cells, without treatment group, normal control group. *** p < 0.001 vs NC group.

Article Snippet: The performance of IDO1-siRNA was validated by western blot using anti-human anti-IDO1 (Sinobiological, China) compared to anti-GAPDH.

Techniques: Animal Model, Staining

IDO1 decreases the expression of chondrogenic genes in the stimulated MSCs. (A) Flow cytometry surface epitope profiling of MSCs. (B) The effect of IDO1 compared to Epacadostat on the chondrogenic differentiation potency of MSCs. (C) The expression of Sox9 under the effect of IDO1 compared to Epacadostat. (D) The expression of COL2A1 under the effect of IDO1 compared to Epacadostat. (E) The expression of the Ihh gene under the effect of IDO1 compared to Epacadostat. (F) The expression of Aggrecan under the effect of IDO1 compared to Epacadostat. (G) The expression of COL2A1 under the effect Epacadostat using IF assay. (H) Epacadostat promotes Sox9 signaling compared to IDO1. *** p < 0.001 and ** p < 0.01 vs NC group.

Journal: Frontiers in Immunology

Article Title: Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model

doi: 10.3389/fimmu.2021.781185

Figure Lengend Snippet: IDO1 decreases the expression of chondrogenic genes in the stimulated MSCs. (A) Flow cytometry surface epitope profiling of MSCs. (B) The effect of IDO1 compared to Epacadostat on the chondrogenic differentiation potency of MSCs. (C) The expression of Sox9 under the effect of IDO1 compared to Epacadostat. (D) The expression of COL2A1 under the effect of IDO1 compared to Epacadostat. (E) The expression of the Ihh gene under the effect of IDO1 compared to Epacadostat. (F) The expression of Aggrecan under the effect of IDO1 compared to Epacadostat. (G) The expression of COL2A1 under the effect Epacadostat using IF assay. (H) Epacadostat promotes Sox9 signaling compared to IDO1. *** p < 0.001 and ** p < 0.01 vs NC group.

Article Snippet: The performance of IDO1-siRNA was validated by western blot using anti-human anti-IDO1 (Sinobiological, China) compared to anti-GAPDH.

Techniques: Expressing, Flow Cytometry

IDO1 regulates the expression of Sox9 through activating β-catenin in the MSCs. (A) Silencing of IDO1 using siRNA in the MSCs. (B) The immunofluorescence expression of Sox9 and β-catenin in the IDO1 silenced cells compared to IDO1-positive cells. (C) The expression of Sox9, COL2A1, and β-catenin by western blot in the IDO1 knocked cells compared to active IDO1. (D) The knocking down of β-catenin in the SF-MSCs using siRNA transfection. (E) The expression of Sox9 under β-catenin silencing in the SF-MSCs compared to active β-catenin using flow cytometry. (F) The expression of Sox9 under β-catenin silencing using confocal laser microscopy. (G) The expression of Sox9 under β-catenin silencing by qPCR. (H) The expression of GSK3β in the MSCs under the effect of IDO1 and Epacadostat compared to the NC group. (I) The expression of GSK3α in the MSCs under the effect of IDO1 and Epacadostat compared to the NC group. (J) The expression of APC in the MSCs under the effect of IDO1 and Epacadostat compared to the NC group. ** p < 0.01 vs NC group. ns, no significance.

Journal: Frontiers in Immunology

Article Title: Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model

doi: 10.3389/fimmu.2021.781185

Figure Lengend Snippet: IDO1 regulates the expression of Sox9 through activating β-catenin in the MSCs. (A) Silencing of IDO1 using siRNA in the MSCs. (B) The immunofluorescence expression of Sox9 and β-catenin in the IDO1 silenced cells compared to IDO1-positive cells. (C) The expression of Sox9, COL2A1, and β-catenin by western blot in the IDO1 knocked cells compared to active IDO1. (D) The knocking down of β-catenin in the SF-MSCs using siRNA transfection. (E) The expression of Sox9 under β-catenin silencing in the SF-MSCs compared to active β-catenin using flow cytometry. (F) The expression of Sox9 under β-catenin silencing using confocal laser microscopy. (G) The expression of Sox9 under β-catenin silencing by qPCR. (H) The expression of GSK3β in the MSCs under the effect of IDO1 and Epacadostat compared to the NC group. (I) The expression of GSK3α in the MSCs under the effect of IDO1 and Epacadostat compared to the NC group. (J) The expression of APC in the MSCs under the effect of IDO1 and Epacadostat compared to the NC group. ** p < 0.01 vs NC group. ns, no significance.

Article Snippet: The performance of IDO1-siRNA was validated by western blot using anti-human anti-IDO1 (Sinobiological, China) compared to anti-GAPDH.

Techniques: Expressing, Immunofluorescence, Western Blot, Transfection, Flow Cytometry, Microscopy

IDO1 decreases the expression of GSK3β in MSCs through inducing its phosphorylation. (A) The immunofluorescence expression of pGSK3β showed increased expression under IDO1 effects, while it was reduced by Epacadostat, suggesting the inhibition of GSK3β phosphorylation by Epacadostat. (B) The western blot expression of pGSK3β under the effect of IDO1 compared to Epacadostat in the MSCs; the effect of two different concentrations showed a change in the expression of phosphorylation protein. (C) The expression of pGSK3β under the effect of IDO1 compared to Epacadostat by flow cytometry. (D) The expression of Wnt1 in the MSCs under the effect of Epacadostat compared to IDO1. (E) The immunofluorescent expression of Wnt1 in the MSCs under the effect of Epacadostat compared to IDO1. (F) The expression of the AhR receptor and β-catenin in the MSCs under the effect of Epacadostat compared to IDO1. *** p < 0.001, and ** p < 0.01.

Journal: Frontiers in Immunology

Article Title: Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model

doi: 10.3389/fimmu.2021.781185

Figure Lengend Snippet: IDO1 decreases the expression of GSK3β in MSCs through inducing its phosphorylation. (A) The immunofluorescence expression of pGSK3β showed increased expression under IDO1 effects, while it was reduced by Epacadostat, suggesting the inhibition of GSK3β phosphorylation by Epacadostat. (B) The western blot expression of pGSK3β under the effect of IDO1 compared to Epacadostat in the MSCs; the effect of two different concentrations showed a change in the expression of phosphorylation protein. (C) The expression of pGSK3β under the effect of IDO1 compared to Epacadostat by flow cytometry. (D) The expression of Wnt1 in the MSCs under the effect of Epacadostat compared to IDO1. (E) The immunofluorescent expression of Wnt1 in the MSCs under the effect of Epacadostat compared to IDO1. (F) The expression of the AhR receptor and β-catenin in the MSCs under the effect of Epacadostat compared to IDO1. *** p < 0.001, and ** p < 0.01.

Article Snippet: The performance of IDO1-siRNA was validated by western blot using anti-human anti-IDO1 (Sinobiological, China) compared to anti-GAPDH.

Techniques: Expressing, Immunofluorescence, Inhibition, Western Blot, Flow Cytometry

IDO1 induces the anti-localization interaction between β-catenin and Sox9 in the MSCs. (A, B) Bioinformatic analysis of P-P interaction between β-catenin and Sox9 using the database of String-db.org and thebiogrid.org. (C) Colocalization assay between β-catenin and Sox9 using immunofluorescence overlapping. (D) The analysis of expression intensity. (E) The calculation of intensity by frequency of every protein expression. (F) The normalizing intensity by calculating Pearson correlation. ** p < 0.01 Epacadostat vs IDO1.

Journal: Frontiers in Immunology

Article Title: Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model

doi: 10.3389/fimmu.2021.781185

Figure Lengend Snippet: IDO1 induces the anti-localization interaction between β-catenin and Sox9 in the MSCs. (A, B) Bioinformatic analysis of P-P interaction between β-catenin and Sox9 using the database of String-db.org and thebiogrid.org. (C) Colocalization assay between β-catenin and Sox9 using immunofluorescence overlapping. (D) The analysis of expression intensity. (E) The calculation of intensity by frequency of every protein expression. (F) The normalizing intensity by calculating Pearson correlation. ** p < 0.01 Epacadostat vs IDO1.

Article Snippet: The performance of IDO1-siRNA was validated by western blot using anti-human anti-IDO1 (Sinobiological, China) compared to anti-GAPDH.

Techniques: Immunofluorescence, Expressing

IDO1 impairs chondrogenic differentiation of MSCs by activating Wnt1/β-catenin through downregulation of miR-122-5p. (A, B) The density and distribution of miRNA in the total RNA obtained from MSCs-treated IDO1 compared to normal. (C) The screening of up-/downregulated miRNA, which shows the downregulation of miR-122-5p under the effect of IDO1. (D) The levels of miR-122-5p in the MSCs treated with Epacadostat compared to IDO1 and NC cells. (E) The target sequence of miR-122-5p in the sequence of the Wnt1 gene. (F) The expression of Wnt1 protein under miR-122-5p mimics compared to miR-122-5p inhibitor. (G) qPCR confirmative experiment for Wnt1 expression under miR-122-5p mimics compared to inhibitor in the MSCs. *** p < 0.001, and ** p < 0.01 vs inhibitor group.

Journal: Frontiers in Immunology

Article Title: Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model

doi: 10.3389/fimmu.2021.781185

Figure Lengend Snippet: IDO1 impairs chondrogenic differentiation of MSCs by activating Wnt1/β-catenin through downregulation of miR-122-5p. (A, B) The density and distribution of miRNA in the total RNA obtained from MSCs-treated IDO1 compared to normal. (C) The screening of up-/downregulated miRNA, which shows the downregulation of miR-122-5p under the effect of IDO1. (D) The levels of miR-122-5p in the MSCs treated with Epacadostat compared to IDO1 and NC cells. (E) The target sequence of miR-122-5p in the sequence of the Wnt1 gene. (F) The expression of Wnt1 protein under miR-122-5p mimics compared to miR-122-5p inhibitor. (G) qPCR confirmative experiment for Wnt1 expression under miR-122-5p mimics compared to inhibitor in the MSCs. *** p < 0.001, and ** p < 0.01 vs inhibitor group.

Article Snippet: The performance of IDO1-siRNA was validated by western blot using anti-human anti-IDO1 (Sinobiological, China) compared to anti-GAPDH.

Techniques: Sequencing, Expressing